I took this photo to a living specimen. This time, it is only one shot, 1/60, F = 11. Canon EF 100 mm, f. 2.8, macro. F 8-11 gives about 5-7 mm DOF. In the field, a flash with a good diffuser is mandatory. Usually not need stack photos if follow f=11, S=1/100-120 (or the maximum speed makes a good sync with the flash). Extra light should be given with ISO or the flash.